The small and middle-size enterprises (SME) are the stabilizing elements in the regional development. This research is oriented at strengths and weaknesses of this kind of enterprising. Small and middle-size entrepreneurs can find some support from regional institutions, first of all from the South Bohemian Chamber of Commerce and the Regional Authority. These institutions provide various counseling, inform about support programs for SME, process projects and create better business conditions. There are possibilities how to increase the market position and the competitiveness of SME. Another possibility for a better competitiveness and position of SME in the region is the establishing of business networks.